There has been some confusion regarding this over the last few months.
In ESX/ESXi 3.5 U2 Jumbo Frames were announced as supported. But the caveat here is that Jumbos are only supported on Virtual Machine networking, NOT storage networking. So tossing Jumbo Frames into the iSCSI adapter either hardware or software puts you into a non-supported configuration. People are going to email me all sorts of things proving it's "supported" but it's not.
http://www.vmware.com/support/vi3/doc/whatsnew_esx35_vc25.html
(Jumbo frames are not supported for NAS and iSCSI traffic. They are limited to data networking only.)
Even when Jumbo Frames are turned on. I haven't noticed a huge performance increase mainly due to the nature of iSCSI. But let's keep this topic on the books, and see what happens when ESX 4.0 comes out.
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